Construction and identification of adenovirus vector for double-mutant human hypoxia-inducible factor-1alpha gene / 南方医科大学学报
Journal of Southern Medical University
;
(12): 445-447, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-268110
ABSTRACT
<p><b>OBJECTIVE</b>To construct an adenovirus vector containing the double-mutant hypoxia-inducible factor-1alpha (HIF-1alpha) gene for exploring the therapeutic angiogenesis for coronary heart disease.</p><p><b>METHODS</b>Human double-mutant HIF-1alpha cDNA was obtained from PCR of pShuttle-2-HIF-1alpha containing the mutant HIF-1alpha gene (564). The recombinant adenoviral plasmid containing mutant HIF-1alpha cDNA was constructed by ligation of recombinant pShuttle2 containing double-mutant HIF-1alpha cDNA and Adeno-X viral DNA, followed by its identification and transfection into adenoviral packaging cells HEK293 via lipofectamine 2000.</p><p><b>RESULT AND CONCLUSION</b>The recombinant pAdeno-HIF-1alpha was correctly constructed and verified by restriction endonuclease and DNA sequencing analyseis. This recombinant adenovirus containing the double-mutant HIF-1alpha gene may facilitate further investigation of mutant HIF-1alphagene therapy for coronary heart disease.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Terapêutica
/
Terapia Genética
/
Adenoviridae
/
Doença das Coronárias
/
Linhagem Celular Tumoral
/
Subunidade alfa do Fator 1 Induzível por Hipóxia
/
Vetores Genéticos
/
Genética
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2007
Tipo de documento:
Artigo
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