Comparative proteomic analysis of the prefrontal cortex between normal and heroin-addicted rats / 南方医科大学学报
Journal of Southern Medical University
;
(12): 427-431, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-268115
ABSTRACT
<p><b>OBJECTIVE</b>To obtain two-dimensional gel electrophoresis maps of the prefrontal cortex (PFC) proteins of normal and heroin-addicted rats for identifying the differentially expressed proteins in the addicted rats.</p><p><b>METHODS</b>Rat models of heroin addiction were established, and the proteins in the PFC underwent two-dimensional gel electrophoresis with immobiline pH gradient isoelectric focusing as the first and vertical SDS-PAGE as the second dimension. ImageMaster 2D 5.0 analysis software, matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) or MALDI-TOF-TOF-MS as well as NCBInr database searching were performed to separate and identify the proteome in the rat PFC.</p><p><b>RESULTS</b>Seven protein spots to represent 5 proteins were identified by PMF and MALDI-TOF-TOF-MS. Among those proteins, glucose-regulated protein (58 kD) and 26 S proteasome subunit p40.5 existed only in heroin-addicted rats, in which ATP synthase D chain was down-regulated. Ndufa10 and Eno1 were present in both groups, but their molecular mass and pI were different.</p><p><b>CONCLUSION</b>Immobilized pH gradient two-dimensional gel electrophoresis allows good reproducibility in separation and identification of the proteome in the PDF of heroin-addicted rats, which facilitates further investigation of pathogenic mechanisms of heroin addiction and central nerve injury.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Processamento de Imagem Assistida por Computador
/
Eletroforese em Gel Bidimensional
/
Reprodutibilidade dos Testes
/
Córtex Pré-Frontal
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
/
Proteoma
/
Proteômica
/
Heroína
/
Eletroforese em Gel de Poliacrilamida
/
Metabolismo
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2007
Tipo de documento:
Artigo
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