Optimization of expression condition of SARS-CoV PUPs genes in E. coli / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1000-1006, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-268539
ABSTRACT
According to previous studies of SARS-CoV (Severe acute respiratory syndrome coronavirus), a variety of novel accessory genes have been identified in SARS-CoV genome, which were interspersed the structural genes of SARS-CoV and considered to be unique to the SARS-CoV genome. The predicted unknown proteins (PUPs) encoded by the accessory genes might play important roles in the SARS-CoV infection. Three of those genes, called X4, X5 and ORF10, were synthesized and introduced into E. coli to induce expression. SDS-PAGE and Western blotting revealed that the three genes have been expressed in E. coli. The induction of SARS PUPs genes expression in different temperatures, induction times, IPTG concentrations and A values of E. coli cells were performed. The optimal induction condition of SARS-CoV PUPs genes was characterized according to the orthorgonal analysis. The ratio of recombinant proteins of PUPs to total proteins is as follows X4, 20%; X5, 27.8%; ORF10, 68.5% under the optimum conditions.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Temperatura
/
Tempo
/
Proteínas Virais
/
Proteínas Recombinantes
/
Regulação Viral da Expressão Gênica
/
Proteínas da Matriz Viral
/
Fases de Leitura Aberta
/
Genoma Viral
/
Coronavírus Relacionado à Síndrome Respiratória Aguda Grave
/
Escherichia coli
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2007
Tipo de documento:
Artigo
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