Chromatographic fingerprint of Cnidium monnieri / 药学学报
Acta Pharmaceutica Sinica
;
(12): 877-881, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-268561
ABSTRACT
To establish the method of HPLC-fingerprint analysis for the quality control of Cnidium monnieri L. Cuss., and identify its active constituents by HPLC-MS, 35 batches of samples were analyzed on a Shimadzu C18 column with a gradient of acetonitrile and 0.1% aqueous aceticacid at a flow rate of 1.0 mL x min(-1) and detected at 245 nm and 322 nm. Furthermore, the typical samples were detected by HPLC-DAD-MS under negative ion mode. 35 batches of Cnidium monnieri L. Cuss. samples were classified into four types based on the results of similarity analysis. According to the comparison of the t(R), MS data and UV maximum absorbance (lambda(max)) values with the standards, 8, 7, 4 and 2 coumarins components were identified in four types of Cnidium monnieri L. Cuss. extracts, separately. The method is repeatable and reliable, and it is capable of effectively controlling the quality of Cnidium monnieri L.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plantas Medicinais
/
Controle de Qualidade
/
Sementes
/
Espectrofotometria Ultravioleta
/
Química
/
Reprodutibilidade dos Testes
/
Cromatografia Líquida de Alta Pressão
/
Sensibilidade e Especificidade
/
Ecossistema
/
Cumarínicos
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2007
Tipo de documento:
Artigo
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