Application of flow cytometry in diagnosis of T-cell rich diffuse large B-cell lymphoma / 中华病理学杂志
Chinese Journal of Pathology
; (12): 29-32, 2007.
Article
em Zh
| WPRIM
| ID: wpr-268849
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the application of flow cytometry in diagnosis of T-cell rich diffuse large B-cell lymphoma.</p><p><b>METHODS</b>Histopathologic features, immunohistochemical findings and flow cytometry results of three cases of T-cell rich diffuse large B-cell lymphoma were reviewed retrospectively.</p><p><b>RESULTS</b>In CD45-side scatter (SSC) dot plot of the first patient, two different CD45-positive lymphoid cell populations were identified. The bright population consisted of both T and B cells, with a T-cell predominance. The dim population consisted mainly of B cells which showed lambda light chain restriction. In the second patient, CD45-positive cells were subdivided into two groups according to CD45-SSC dot plot. The small population consisted of both T and B cells, with a T-cell predominance. The large population consisted mainly of B cells which showed kappa light chain restriction. In the third patient, CD19-positive cells were subdivided into two groups according to the expression of CD20 in CD19-CD20 dot plot. The CD20-positive population expressed both kappa and lambda light chains, while the CD20-negative population demonstrated kappa light chain restriction.</p><p><b>CONCLUSIONS</b>Neoplastic B cells can be distinguished from reactive lymphoid cells in T-cell rich diffuse large B-cell lymphoma by flow cytometry, according to a number of parameters which include intensity of antigen expression, loss of antigens, expression of non-B-cell lineage antigens, patterns of forward scatter (FSC) and/or SSC, and expression of immature B-cell antigens.</p>
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Índice:
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Assunto principal:
Patologia
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Imuno-Histoquímica
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Linfócitos T
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Linfoma Difuso de Grandes Células B
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Cadeias lambda de Imunoglobulina
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Antígenos Comuns de Leucócito
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Antígenos CD20
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Antígenos CD19
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Diagnóstico
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Diagnóstico Diferencial
Tipo de estudo:
Diagnostic_studies
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Prognostic_studies
Limite:
Aged
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Pathology
Ano de publicação:
2007
Tipo de documento:
Article