Effect of lentiviral vector-mediated short hairpin RNA targeting survivin against endometriosis-like lesions in chick embryo chorioallantoic membrane / 南方医科大学学报
Journal of Southern Medical University
;
(12): 995-999, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-268947
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the inhibitory effect of lentiviral vector-mediated short hairpin RNA targeting survivin (LV-survivin shRNA) on angiogenesis and growth of endometriosis-like lesions in chick en embryo chorioallantoic membrane.</p><p><b>METHODS</b>Eutopic endometrium from women with endometriosis was transplanted onto the non-vascular region of (CAM), where LV-survivin shRNA was delivered subsequently. The angiogenesis and the growth of endometriosis-like lesions in the CAM model were evaluated.</p><p><b>RESULTS</b>The angiogenesis and formation of endometriosis-like lesions were significantly suppressed in the CAM model by treatment with LV-survivin shRNA in comparison with those in the untreated CAM models and models treated with empty LV or DMEM (P<0.001). LV-survivin shRNA also caused a significantly higher cell apoptotic rate in the endometriosis-like lesions than the other treatments (P<0.001) and induced necrosis in the lesions.</p><p><b>CONCLUSION</b>LV-survivin shRNA can effectively inhibit angiogenesis induced by the eutopic endometrium and markedly suppress the formation of endometriosis-like lesions in the CAM model.</p>
Texto completo:
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Lentivirus
/
RNA Interferente Pequeno
/
Interferência de RNA
/
Membrana Corioalantoide
/
Modelos Animais de Doenças
/
Endometriose
/
Proteínas Inibidoras de Apoptose
/
Vetores Genéticos
/
Genética
Limite:
Animais
/
Feminino
/
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2012
Tipo de documento:
Artigo
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