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Construction of an anti-tumor immunogene vaccine pVAX-IL-12-GB and its expression in vivo and in vitro / 南方医科大学学报
Journal of Southern Medical University ; (12): 760-765, 2012.
Artigo em Chinês | WPRIM | ID: wpr-269002
ABSTRACT
<p><b>OBJECTIVE</b>To construct a novel immunogene therapeutic plasmid that expresses human interleukin-12 (IL-12), granulocyte-macrophage colony stimulating factor (GM-CSF) and B7.1 and observe its expression in vivo and in vitro.</p><p><b>METHODS</b>Human IL-12 gene fragment was cloned into the upper stream of IRES gene in the previously constructed plasmid pVAX-IRES-GM-CSF-B7.1, and the positive recombinant plasmid pVAX-IL-12-GB was transfected into 293T cells via Lipofectamine 2000. The expressions of IL-12 and GM-CSF-B7.1 mRNA and proteins in the transfected cells were assayed by RT-PCR and ELISA, and B7.1 expression was tested by fluorescence-activated cell sorting and immunofluorescence assay. The plasmid pVAX-IL-12-GB was delivered into mouse muscle by electroporation, and the expression of IL-12 in the muscle tissue was identified by immunohistochemistry.</p><p><b>RESULTS</b>Enzyme digestion, PCR and sequence analysis all confirmed successful construction of the recombinant plasmid pVAX-IL-12-GB. IL-12, GM-CSF and B7.1 expressions were all detected in transfected 293T cells, and the expression of IL-12 was also detected in the transfected mouse muscular tissues.</p><p><b>CONCLUSION</b>A novel anti-tumor immunogene vaccine constructed can be expressed both in vivo and in vitro, which facilitates further studies of tumor immunogene therapy.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Plasmídeos / Transfecção / Terapia Genética / Fator Estimulador de Colônias de Granulócitos e Macrófagos / Eletroporação / Antígeno B7-1 / Interleucina-12 / Vacinas Anticâncer / Alergia e Imunologia / Genética Tipo de estudo: Estudo prognóstico Limite: Animais / Humanos Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2012 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Plasmídeos / Transfecção / Terapia Genética / Fator Estimulador de Colônias de Granulócitos e Macrófagos / Eletroporação / Antígeno B7-1 / Interleucina-12 / Vacinas Anticâncer / Alergia e Imunologia / Genética Tipo de estudo: Estudo prognóstico Limite: Animais / Humanos Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2012 Tipo de documento: Artigo