Effects of arsenic trioxide on the autoimmunity and survival time in BXSB lupus mice / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 138-141, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-269105
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of arsenic trioxide (ATO) on the autoimmunity and survival time in BXSB lupus mice.</p><p><b>METHODS</b>The model BXSB lupus mice were randomly divided into two groups equally, the ATO treated group and the control group, 17 in each group. Mice in the ATO group were given intraperitoneal injection of ATO at the daily dose of 0.4 mg/kg, once every other day for 105 days or 90 days, respectively, and the observation lasted for 210 days. The survival time between the two groups was compared; the serum levels of anti-dsDNA and IgG were detected by enzyme-linked immunosorbent assay (ELISA), and the interferon-gamma (IFN-gamma) mRNA expression in renal and spleen tissue were measured by reverse transcriptase polymerase chain reaction (RT-PCR) technique.</p><p><b>RESULTS</b>Till the 210th day, the total number of death was 8 in the ATO treated group and 13 in the control group, comparison between the two groups showed significantly different (chi2 = 4.20, P < 0.05). The mean OD value of serum anti-dsDNA antibody was lower in the ATO group (0.392 +/- 0.087) than that in the control group (0.566 +/- 0.080, P < 0.001). The serum level of IgG on day 105 in the ATO group was significantly lower than that in the control group and before treatment (P < 0.05). The expression of IFN-gamma mRNA in spleen tissue and renal tissue in the ATO group and the control group was 0.540 +/- 0.300 and 0.338 +/- 0.163, 2.320 +/- 0.522 and 0.588 +/- 0.104 (P < 0.05 and P < 0.01 respectively).</p><p><b>CONCLUSION</b>ATO can prolong the survival time of BXSB lupus mice, decrease the peripheral level of anti-dsDNA antibody and IgG expression, inhibit the over-expression of IFN-gamma mRNA in spleen and kidney tissues.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Óxidos
/
Arsenicais
/
Baço
/
Sangue
/
Imunoglobulina G
/
RNA Mensageiro
/
Ensaio de Imunoadsorção Enzimática
/
Distribuição Aleatória
/
Autoimunidade
/
Análise de Sobrevida
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Ano de publicação:
2007
Tipo de documento:
Artigo
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