Signal transduction pathway in liver fibrosis regulated by human hepatocyte growth factor / 南方医科大学学报
Journal of Southern Medical University
;
(12): 431-434, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-269536
ABSTRACT
<p><b>OBJECTIVE</b>To explore the changes in the gene expression profiles in HepG2 cells transfected by human hepatocyte growth factor (hHGF) and analyze the signal transduction pathway in liver fibrosis regulated by hHGF.</p><p><b>METHOD</b>A 20,000 gene cDNA microarray (Affymetrix) was used to examine the gene expressions in the HepG2 cells transfected by hHGF. The differentially expressed genes were identified and some genes with possible contribution to hepatic fibrosis were subjected to real-time PCR analysis.</p><p><b>RESULT</b>The differentially expressed genes were mostly transcription regulatory molecules, cytokines, signal transduction, glucose metabolism, lipid metabolism. The results of real-time PCR showed up-regulated STAT1 and MAPK1 expression in the cells as were consistent with genechip analysis results.</p><p><b>CONCLUSION</b>hHGF gene transfection results in the gene expression profile changes in HepG2 cells. HGF may regulate liver fibrosis via the JAK/STAT and MAPK pathways.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Transfecção
/
Transdução de Sinais
/
Regulação da Expressão Gênica
/
Fator de Crescimento de Hepatócito
/
Proteína Quinase 1 Ativada por Mitógeno
/
Perfilação da Expressão Gênica
/
Fator de Transcrição STAT1
/
Janus Quinases
/
Células Hep G2
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2010
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS