Cloning and sequence analysis of MYB transcriptional regulator SmP gene of Saussurea medusa Maxim / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 368-371, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-270051
ABSTRACT
A full-length cDNA encoding a MYB-related regulatory gene was isolated from a cDNA library prepared from mRNAs of the red line callus of S. medusa by TD-PCR. The cDNA, designated SmP, is 969 nucleotides long and has an open reading frame of 771 bp with a deduced amino acid sequence of 256 residues. The putative protein of SmP has two typical conversed R2R3-Myb DNA-binding domains in N-terminal and displays a rather high degree of similarity to OsMYB from rice and LBMI from tobacco, showing 73% and 70% identity within the DNA-binding domains. However, the C-terminal domain of the SmP protein does not show obvious similarity to any other known protein sequence. It is rich in hydrophilic amino acids, especially in serine residues (18.38%), partly organized in homopolymeric stretches, a feature often found in activation domain of transcription factors.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Proteínas de Plantas
/
Fatores de Transcrição
/
Dados de Sequência Molecular
/
Química
/
Reação em Cadeia da Polimerase
/
Sequência de Aminoácidos
/
Classificação
/
Clonagem Molecular
/
Homologia de Sequência de Aminoácidos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2003
Tipo de documento:
Artigo
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