Biosynthesis of poly (3-mercaptopropionate) and poly (3-mercaptopropionate-co-3-hydroxybutyrate) with recombinant Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 195-199, 2003.
Artigo
em Inglês
| WPRIM
| ID: wpr-270114
ABSTRACT
Polythioesters newly emerged as a type of novel polymer and they have showed great potential for application in industries. In this study, genes of butyrate kinase (buk) and phosphotransbutyrylase (ptb) from Clostridium acetobutylicum, and poly (3-hydroxybutyrate) (PHB) synthase gene from Thiocapsa pfennigii were used for construction of a metabolic pathway to synthesize the polythioesters. When 3-mercaptopropionate and 3-hydroxybutyrate were fed, poly (3-mercaptopropoinate) [poly (3MP)] and poly(3-mercaptopropionate-co-3-hydroxybutyrate) [poly(3MP-co-3HB)] were synthesized by recombinant Escherichia coli JM109 (pBPP1) harboring the constructed metabolic pathway. Results indicated clearly that all these genes are necessary for the synthesis of poly(3MP) and poly(3MP-co-3HB).
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosfato Acetiltransferase
/
Plasmídeos
/
Polímeros
/
Espectrofotometria Infravermelho
/
Aciltransferases
/
Química
/
Fosfotransferases (Aceptor do Grupo Carboxila)
/
Ácido 3-Hidroxibutírico
/
Escherichia coli
/
Genética
Idioma:
Inglês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2003
Tipo de documento:
Artigo
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