Construction and identification of Ksp-cadherin-Gpx1-Klk1 expression vector / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1327-1330, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-270149
ABSTRACT
<p><b>OBJECTIVE</b>To construct a Gpx1 and klk1 recombinant vector containing the kidney-specific promoter Ksp-cadherin.</p><p><b>METHODS</b>Human Gpx1, Klk1 and Ksp-cadherin cDNAs were amplified with PCR and inserted in a stepwise manner into the expressive vector pIRES-EGFP to construct the recombinant vector Ksp-cadherin-Gpx1-Klk1. The constructed vector was verified with restriction enzyme digestion and sequence analysis.</p><p><b>RESULTS AND CONCLUSION</b>The recombinant expression vector Ksp-cadherin-Gpx1-Klk1 was constructed and identified successfully, which provides a potent tool for preparing transgenic animals to investigate gene therapy for ischemia-reperfusion injury in kidney transplantation.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Calicreínas
/
Terapia Genética
/
Caderinas
/
Regiões Promotoras Genéticas
/
Clonagem Molecular
/
Vetores Genéticos
/
Genética
/
Glutationa Peroxidase
/
Rim
/
Metabolismo
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2008
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS