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Protective effects of estrogen on mitochondria in human umbilical vascular endothelial cells / 南方医科大学学报
Journal of Southern Medical University ; (12): 1154-1156, 2008.
Artigo em Chinês | WPRIM | ID: wpr-270188
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the protective effects of estrogen on the mitochondria in human umbilical vascular endothelial cells (HUVECs).</p><p><b>METHODS</b>HUVECs were exposed to H2O2 at 250 micromol/L for 4 h with or without pretreatment with 17-estradiol (E2) and ICI182780. Complex IV activity of the cells was measured with chromometry, and 2, 7-dichlorofluorescein diacetate (DCFH-DA) was used to determine intracellular reactive oxygen species (ROS). Intracellular adenosine triphosphate (ATP) level was quantified with a luciferin- and luciferase-based assay.</p><p><b>RESULTS</b>Compared to the blank control group, H2O2 caused a decrease in complex IV activity, intracellular ATP level, and the cell viability, but elevated intracellular ROS. E2 pretreatment of cells significantly attenuated these effects of H2O2 exposure. ICI182780 administered prior to E2 pretreatment antagonized the protective effects of E2 against H2O2 exposure.</p><p><b>CONCLUSION</b>E2 offers mitochondrial protective effects on HUVECs, which is mediated by the estrogen receptors.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Veias Umbilicais / Células Cultivadas / Espécies Reativas de Oxigênio / Complexo IV da Cadeia de Transporte de Elétrons / Estresse Oxidativo / Citoproteção / Biologia Celular / Células Endoteliais / Estrogênios Limite: Feminino / Humanos / Gravidez Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2008 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Veias Umbilicais / Células Cultivadas / Espécies Reativas de Oxigênio / Complexo IV da Cadeia de Transporte de Elétrons / Estresse Oxidativo / Citoproteção / Biologia Celular / Células Endoteliais / Estrogênios Limite: Feminino / Humanos / Gravidez Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2008 Tipo de documento: Artigo