High-performance liquid chromatography-mass spectrometry for determining olmesartan in human plasma / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To establish a simple and rapid HPLC-MS method for determining the contents of olmesartan in human plasma.</p><p><b>METHODS</b>Plasma were precipitated with trifluoroacetic acid, then analyzed on an HyPurity C(18) column (150 mm 2.1 mm, 5 microm). Samples at 40 degrees celsius;. The mobile phase consisted of water-methanol- acetonitrile(146026) with a flow rate of 0.22 ml/min.</p><p><b>RESULTS</b>The lower limit of qualification was 25 microg/L. The calibration curve was linear over the range of 25-3200 microg/L (r=0.9998), with the intra-day and inter-day RSD less than 15%.</p><p><b>CONCLUSION</b>The method is sensitive, rapid and suitable for the study of pharmacokinetics and bioavailability of olmesartan.</p>