Screening peptides binding specifically to large intestinal cancer LoVo cells from phage random peptide library / 南方医科大学学报
Journal of Southern Medical University
; (12): 986-990, 2008.
Article
em Zh
| WPRIM
| ID: wpr-270229
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To screen the polypeptides specifically binding to human large intestinal cancer LoVo cells from a phage-displayed peptide library for potential use as targeting vectors for large intestinal cancer therapy.</p><p><b>METHODS</b>With the LoVo cells as the target cells and human normal large intestinal mucosal epithelial cells as the absorber cells for subtraction biopanning from a c7c phage-display peptide library, the positive phage clones were identified by enzyme-linked immunosorbent assay (ELISA) and immunofluorescence detection. The amino acid sequences of the identified peptides were deduced by DNA sequencing.</p><p><b>RESULTS</b>After 3 rounds of screening, 5 positive phage clones showing specific binding to LoVo cells and containing conserved motif RPMP were obtained from the 20 randomly selected clones.</p><p><b>CONCLUSION</b>Specific peptide against large intestinal cancer cells can be obtained from a phage-display peptide library for use as potential vectors for targeting therapy of large intestinal cancer.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Patologia
/
Peptídeos
/
Ligação Proteica
/
Ligação Competitiva
/
Dados de Sequência Molecular
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Sequência de Bases
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Neoplasias Colorretais
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Sequência de Aminoácidos
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Biblioteca de Peptídeos
/
Linhagem Celular Tumoral
Tipo de estudo:
Clinical_trials
/
Diagnostic_studies
/
Screening_studies
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2008
Tipo de documento:
Article