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The in vitro myelin formation in neurospheres of human neural stem cells / 中华创伤杂志(英文版)
Chinese Journal of Traumatology ; (6): 341-345, 2003.
Artigo em Inglês | WPRIM | ID: wpr-270300
ABSTRACT
<p><b>OBJECTIVE</b>To explore the culture conditions of human neural stem cells and to investigate the ultrastructure of neurospheres.</p><p><b>METHODS</b>The cells from the embryonic human cortices were mechanically dissociated. N2 medium was adapted to culture and expand the cells. The cells were identified by immunocytochemistry and EM was applied to examine the ultrastructure of neurospheres.</p><p><b>RESULTS</b>The neural stem cells from human embryonic brains were successfully cultured and formed typical neurospheres in suspension, and most of the cells expressed vimentin, which was a marker for neural progenitor cells, and the cells could differentiate into neurons, astrocytes and oligodendrocytes. In vitro myelin formation in neurospheres were observed at an early stage of culture.</p><p><b>CONCLUSIONS</b>Human neural stem cells can be cultured from embryonic brains, can form the typical neurospheres in suspension in vitro and have the ability of myelinating, and may be potential source for transplantation in treating myelin disorders.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Fisiologia / Células-Tronco / Encéfalo / Imuno-Histoquímica / Microscopia Eletrônica / Células Cultivadas / Sensibilidade e Especificidade / Meios de Cultura / Biologia Celular Tipo de estudo: Estudo diagnóstico Limite: Feminino / Humanos / Masculino Idioma: Inglês Revista: Chinese Journal of Traumatology Ano de publicação: 2003 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Fisiologia / Células-Tronco / Encéfalo / Imuno-Histoquímica / Microscopia Eletrônica / Células Cultivadas / Sensibilidade e Especificidade / Meios de Cultura / Biologia Celular Tipo de estudo: Estudo diagnóstico Limite: Feminino / Humanos / Masculino Idioma: Inglês Revista: Chinese Journal of Traumatology Ano de publicação: 2003 Tipo de documento: Artigo