Effect of adenovirus-mediated gene transfection of vascular endothelial growth factor on survival of random flaps in rats / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 199-204, 2003.
Artigo
em Inglês
| WPRIM
| ID: wpr-270332
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect of local application of vascular endothelial growth factor (VEGF) via adenovirus-mediated gene transfer on survival of full thickness flaps selected randomly in rats.</p><p><b>METHODS</b>Thirty Sprague-Dawley rats weighing 480-520 g were used in this study. A dorsal flap (8 cm x 2 cm) in full thickness with the pedicle located at the level of the iliac crest was designed. Then the rats received 1,012 pfu replication-deficient recombinant adenovirus carrying VEGF (AdCMV-VEGF group, n=10), 1,012 pfu recombinant beta-galactosidase adenovirus (AdCMV-Gal group, n=10) and 1 ml saline (saline group, n=10), respectively, in the distal two thirds of the proposed flap by means of subdermal injection at 8 different locations. Three days after treatment, the flaps were elevated as originally designed and sutured back in situ. The survival rate of the flaps was evaluated on day 7 after operation.</p><p><b>RESULTS</b>The survival rate of the flaps in the AdCMV-VEGF group increased significantly as compared with those of the AdCMV-Gal group (P<0.01) and the saline group (P<0.01). Immunohistochemical staining showed that VEGF was expressed in the survival flaps injected with AdCMV-VEGF. Histological analysis showed that more granulation tissues and angiogenesis were observed in the AdCMV-VEGF group than those in the AdCMV-Gal and the saline groups.</p><p><b>CONCLUSIONS</b>Local application of adenovirus-mediated VEGF165 cDNA may efficiently improve the survival of ischemic skin flaps.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Retalhos Cirúrgicos
/
Transfecção
/
Terapia Genética
/
Adenoviridae
/
Fatores de Crescimento Endotelial
/
Linfocinas
/
Ratos Sprague-Dawley
/
Neovascularização Fisiológica
/
Peptídeos e Proteínas de Sinalização Intercelular
/
Fatores de Crescimento do Endotélio Vascular
Limite:
Animais
Idioma:
Inglês
Revista:
Chinese Journal of Traumatology
Ano de publicação:
2003
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS