Effect of magnesium-free on glucocorticoid receptor expression in primary cultured cortical neurons of fetal rats in vitro / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 211-214, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-270386
ABSTRACT
<p><b>OBJECTIVE</b>To study the changes of glucocorticoid receptor (GR) expression in embryonic rat cortical neurons exposed to transient Mg(2+)-free treatment.</p><p><b>METHODS</b>Six days after rat cortical neuronal cultures, two groups were created based on the medium to which were transiently exposed. The control group was exposed to a physiological solution (PS), and the Mg(2+)-free group was exposed to the same medium as the control group except for the removal of magnesium. The expression of GR mRNA and protein was determined by real-time PCR and immunocytochemistry staining 1, 7 and 12 days after transient Mg(2+)-free treatment.</p><p><b>RESULTS</b>Compared to the control group, the Mg(2+)-free group displayed the significantly less accumulated optical density (AOD) of GR immunoreactivity 12 days after transient Mg(2+)-free treatment (p<0.05). On the contrary, GR mRNA expression increased significantly 1 and 7 days after transient Mg(2+)-free treatment in the Mg(2+)-free group (p<0.05).</p><p><b>CONCLUSIONS</b>GR expression is modified following Mg-free-induced injury in cultured developing neurons in rats.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Sistema Hipófise-Suprarrenal
/
RNA Mensageiro
/
Receptores de Glucocorticoides
/
Sobrevivência Celular
/
Células Cultivadas
/
Córtex Cerebral
/
Ratos Wistar
/
Feto
/
Genética
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Contemporary Pediatrics
Ano de publicação:
2010
Tipo de documento:
Artigo
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