Effects of garlic oil combined with resveratrol on inducting of apoptosis and expression of Fas, bcl-2 and bax in human gastric cancer cell line / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 39-42, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-270460
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of garlic oil combined with resveratrol on the apoptosis and expression of Fas, bcl-2 and bax in human gastric cancer cell line MGC-803.</p><p><b>METHODS</b>The experiment included three groups which were the control group, the combined medicine group 1 (including 25 microg/ml oil garlic and 25 microg/ml resveratrol) and the combined medicine group 2 (including 50 microg/ml oil garlic and 50 microg/ml resveratrol). The apoptosis of cell was examined by DNA gel electrophoresis and flow cytometry for annexin v; the expression of Fas was determined by flow cytometry at the 24th hour after the treatment; the mRNA expression of bcl-2 and Bax gene were measured by RT-PCR method at the 24th ang 48th after treatment, respectively.</p><p><b>RESULTS</b>The garlic oil combined with resveratrol induced cell apoptosis markedly at the 24th after the treatment The protein expression of Fas in the combined medicine groups was 10.59% and 14.16% respectively. As compared with the control group (5.27%), the statistical significance was obvious. The mRNA level of Bax was elevated significantly, however the mRNA expression of bcl-2 was decreased at the 24th and 48th after the treatment.</p><p><b>CONCLUSIONS</b>The garlic oil combined with the resveratrol might obviously induce the apoptosis of gastric cancer cell line MGC-803 which be involved in increasing the expression of Fas protein and bax gene and decreasing the expression of bcl-2 gene at the same time.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Estilbenos
/
Neoplasias Gástricas
/
Óleos de Plantas
/
RNA Mensageiro
/
Regulação Neoplásica da Expressão Gênica
/
Apoptose
/
Receptor fas
/
Proteínas Proto-Oncogênicas c-bcl-2
/
Genes bcl-2
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Preventive Medicine
Ano de publicação:
2008
Tipo de documento:
Artigo
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