S phase cell percentage normalized BrdU incorporation rate, a new parameter for determining S arrest / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 215-219, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-270611
ABSTRACT
In this study, a new parameter, S phase cell percentage (S fraction) normalized BrdU (SFN-BrdU) incorporation rate, was introduced to detect S arrest. The results showed a positive linear correlation between the BrdU incorporation rate and the S fraction in unperturbed 16HBE cells. Theoretical analysis indicated that only S arrest could result in a decrease in the SFN-BrdU incorporation rate. Additionally, the decrease in SFN-BrdU incorporation rate and the activation of DNA damage checkpoints further demonstrated that S arrest was induced by diethyl sulfate treatment of 16HBE cells. In conclusion, SFN-BrdU incorporation rate can be used to detecting S arrest.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Dano ao DNA
/
Bromodesoxiuridina
/
Farmacocinética
/
Fase S
/
Biologia Celular
/
Proliferação de Células
/
Células Epiteliais
/
Pontos de Checagem da Fase S do Ciclo Celular
Limite:
Humanos
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2014
Tipo de documento:
Artigo
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