Three-step purification of preparative-scale antiCD20 (Fab')2 / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 622-625, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-270635
ABSTRACT
<p><b>OBJECTIVE</b>To establish a three-step purification method of preparative-scale antiCD20 (Fab')2 using AKTA prime.</p><p><b>METHODS</b>AntiCD20 (Fab')2 was extracted by hyperosmotic solution and then purified by CM sepharose FF, phenyl sepharose FF, and protein G sepharose FF.</p><p><b>RESULTS</b>Around 8 mg anti-CD20 (Fab')2, whose purification was 96.678%, was purified. The antigen-binding activity of antiCD20 (Fab')2 was similar to that of antiCD20 (Fab')2 purified by protein G sepharose FF and S-100.</p><p><b>CONCLUSION</b>The three-step purification method can obtain high-purity preparative-scale antiCD20 (Fab')2 in a simple way.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fragmentos Fab das Imunoglobulinas
/
Cromatografia
/
Antígenos CD20
/
Alergia e Imunologia
/
Métodos
/
Anticorpos
Limite:
Humanos
Idioma:
Chinês
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2008
Tipo de documento:
Artigo
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