Application of surface-enhanced laser desorption/ionization time-of-flight-based serum proteomic array technique for the early diagnosis of prostate cancer / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 45-51, 2006.
Artigo
em Inglês
| WPRIM
| ID: wpr-270825
ABSTRACT
<p><b>AIM</b>To identify the serum biomarkers of prostate cancer (PCa) by protein chip and bioinformatics.</p><p><b>METHODS</b>Serum samples from 83 PCa patients and 95 healthy men were taken from a mass screening in Changchun, China. Protein profiling was carried out using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS). The data of spectra were analyzed using two bioinformatics tools.</p><p><b>RESULTS</b>Eighteen serum differential proteins were identified in the PCa group compared with the control group (P < 0.01). There were four proteins at the higher serum level and 14 proteins at the lower serum level in the PCa group. A decision tree classification algorithm that used an eight-protein mass pattern was developed to correctly classify the samples. A sensitivity of 92.0% and a specificity of 96.7% for the study group were obtained by comparing the PCa and control groups.</p><p><b>CONCLUSION</b>We identified new serum biomarkers of PCa. SELDI-TOF MS coupled with a decision tree classification algorithm will provide a highly accurate and innovative approach for the early diagnosis of PCa.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Neoplasias da Próstata
/
Sangue
/
Informática Médica
/
Árvores de Decisões
/
Biomarcadores
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
/
Proteoma
/
Diagnóstico
/
Métodos
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
/
Estudo de rastreamento
Limite:
Idoso
/
Aged80
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Asian Journal of Andrology
Ano de publicação:
2006
Tipo de documento:
Artigo
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