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Expression of a novel beta adaptin subunit mRNA splice variant in human testes / 亚洲男科学杂志(英文版)
Asian Journal of Andrology ; (6): 179-188, 2005.
Artigo em Inglês | WPRIM | ID: wpr-270855
ABSTRACT
<p><b>AIM</b>To identify a novel isoform of adaptin 2 beta subunit (named Ap2beta-NY) and to investigate its relationship with testicular development and spermatogenesis.</p><p><b>METHODS</b>Using a human testis cDNA microarray, a clone (Ap2beta-NY), which was strongly expressed in adult testes but weakly expressed in embryo testes, was sequenced and analyzed. Using polymerase chain reaction (PCR), the tissue distribution and expression time pattern of Ap2beta-NY were determined.</p><p><b>RESULTS</b>Ap2beta-NY was identified and has been deposited in the GenBank (AY341427). The expression level of Ap2beta-NY in the adult testis was about 3-fold higher than that in the embryo testis. PCR analysis using multi-tissue cDNA indicated that Ap2beta-NY was highly expressed in the testis, spleen, thymus, prostate, ovary, blood leukocyte and brain, but not in the heart, placenta, lung, liver, skeletal muscle, kidney and pancreas. In addition, Ap2beta-NY was variably expressed in the testes of patients with spermatogenesis-disturbance and spermatogenesis-arrest but not expressed in those of Sertoli-cell-only syndrome, which implied that, in the testis, Ap2beta-NY was restrictively expressed in germ cells.</p><p><b>CONCLUSION</b>Ap2beta-NY is an isoform of Ap2beta and may be involved in regulating the process of spermatogenesis and testis development.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Espermatogênese / Testículo / RNA Mensageiro / Dados de Sequência Molecular / Sequência de Bases / Química / Splicing de RNA / Reação em Cadeia da Polimerase / Sequência de Aminoácidos / DNA Complementar Limite: Humanos / Masculino Idioma: Inglês Revista: Asian Journal of Andrology Ano de publicação: 2005 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Espermatogênese / Testículo / RNA Mensageiro / Dados de Sequência Molecular / Sequência de Bases / Química / Splicing de RNA / Reação em Cadeia da Polimerase / Sequência de Aminoácidos / DNA Complementar Limite: Humanos / Masculino Idioma: Inglês Revista: Asian Journal of Andrology Ano de publicação: 2005 Tipo de documento: Artigo