Lentiviral vector-mediated RNA interfere gene Nogo receptor to repair spinal cord injury / 中华外科杂志
Chinese Journal of Surgery
;
(12): 1573-1576, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-270914
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effects of lentiviral vector-mediated RNA interfere gene Nogo receptor (NgR) of rat cortical neurons in repairing spinal cord injury.</p><p><b>METHODS</b>The recombinant-lentiviral vector with small inferring RNA siNgR199 which had been constructed was transfected into rat cortical neuron cells in vitro in 3 multiplicity of infection (MOI). The infection rate was determined with fluorescent microscope, and the target gene was detected by PCR analysis. Then, the recombinant was injected into the cortical motor area of the rats with severe spinal cord injury, and the saline was also injected into other rats with severe spinal cord injury as a match control. The functional recovery of the rats' hindlimb was assessed using BBB score and the nerve fiber of the injured region was observed by nerve tracing.</p><p><b>RESULTS</b>The rate of recombinant infecting rat cortical neuron in vitro exceeded 99%. PCR analysis confirmed that the effect of lentiviral vector-mediated RNA interfering gene NgR of rat cortical neurons in vitro was 61%. Although all rats with spinal cord injury were observed to have the hindlimb functional recovery, these rats injected with recombinant had better hindlimb functional recovery than others showing by more BBB score (P < 0.01). Moreover, it was found that some nerve fiber passed the injured spinal cord region of the rats which were injected with recombinant.</p><p><b>CONCLUSION</b>The recombinant lentiviral vector with siNgR199 which had been constructed is able to promote the growth of nerve fiber and the functional recovery of the rats' hindlimb.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Traumatismos da Medula Espinal
/
Terapêutica
/
Transfecção
/
Células Cultivadas
/
Ratos Sprague-Dawley
/
Receptores de Peptídeos
/
Lentivirus
/
Receptores de Superfície Celular
/
Interferência de RNA
/
Modelos Animais de Doenças
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Surgery
Ano de publicação:
2010
Tipo de documento:
Artigo
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