Enhancement of the bystander effect by tanshinone IIA in HSV-tK/GCV system is related to expression of connexin 43 mRNA / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 146-149, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-271032
ABSTRACT
<p><b>OBJECTIVE</b>To investigate enhancement of the bystander effect by tanshinone IIA (Tan) in HSV-tK/GCV system and the correlation with expression of connexin 43 mRNA.</p><p><b>METHODS</b>The cytotoxic effect in HSV-tK/GCV in cervical carcinoma cell line ME180 (ME) and ME/TK was examined by MTT assays. Cx43 mRNA expression was detected by fluor-quantitative RT-PCR.</p><p><b>RESULTS</b>Tan markedly increased sensitivity of ME/TK cells for GCV in HSV-tK/GCV system. In the presence of 2 micro g/ml GCV, compared with the absence of Tan (0 mol/L), an obvious decrease in survival rate was seen at any given mixture of ME and ME/TK cells exposed to 1.3 x 10(-9) mol/L Tan. Statistics showed significant difference (P < 0.05). However, enhancement of bystander mediated cell killing occurred only in the range of Tan concentrations used (1.3 x 10(-8), 1.3 x 10(-9) mol/L). RT-PCR showed that the ratio of relative copy number of Cx43 mRNA increased by 8.83 and 8.47-fold in ME cells exposed to 1.3 x 10(-8) and 1.3 x 10(-9) mol/L Tan, respectively.</p><p><b>CONCLUSION</b>For the first time we report that in cervical carcinoma ME180 cell line, Tan possesses a remarkable enhancing role on the bystander effect in the HSV-tK/GCV system. It is associated with up-regulation of Cx43 mRNA expression.</p>
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DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fenantrenos
/
Terapêutica
/
Timidina Quinase
/
Terapia Genética
/
Ganciclovir
/
Sobrevivência Celular
/
Neoplasias do Colo do Útero
/
Simplexvirus
/
Conexina 43
Limite:
Feminino
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Oncology
Ano de publicação:
2004
Tipo de documento:
Artigo
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