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Antisense DNMT1 gene fragment in the sensitivity change of SMMC-7721 cells to tumor necrosis factor related apoptosis inducing ligand and its mechanism / 中华肿瘤杂志
Chinese Journal of Oncology ; (12): 538-541, 2003.
Artigo em Chinês | WPRIM | ID: wpr-271086
ABSTRACT
<p><b>OBJECTIVE</b>To observe the sensitivity change of SMMC-7721 cells transfected with antisense DNMT1 gene fragment to tumor necrosis factor related apoptosis inducing ligand (TRAIL) and its mechanism.</p><p><b>METHODS</b>Cell survival rate was measured by trypan blue, apoptosis rate by TUNEL method and the expression of bcl-2, bax and bad by flow cytometry.</p><p><b>RESULTS</b>Cell survival rate of SMMC-7721 cells transfected with antisense DNMT1 gene fragment was markedly lower than that transfected with sense DNMT1 gene fragment or empty vector (P < 0.05 and 0.01), but the apoptosis rate was on the contrary (P < 0.05 or 0.01). The expression of bax and bad (especially the former), but not bcl-2 of SMMC-7721 cells transfected with antisense DNMT1 gene fragment was markedly higher than those of SMMC-7721 cells transfected with sense DNMT1 gene fragment or empty vector.</p><p><b>CONCLUSION</b>The sensitivity of SMMC-7721 cells to TRAIL can be enhanced by the transfection of antisense DNMT1 gene fragment, which may be related to the increase of bax and bad expression.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Farmacologia / Glicoproteínas de Membrana / Proteínas de Transporte / Elementos Antissenso (Genética) / Proteínas Proto-Oncogênicas / Fator de Necrose Tumoral alfa / Apoptose / Proteínas Proto-Oncogênicas c-bcl-2 / Linhagem Celular Tumoral Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Oncology Ano de publicação: 2003 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Farmacologia / Glicoproteínas de Membrana / Proteínas de Transporte / Elementos Antissenso (Genética) / Proteínas Proto-Oncogênicas / Fator de Necrose Tumoral alfa / Apoptose / Proteínas Proto-Oncogênicas c-bcl-2 / Linhagem Celular Tumoral Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Oncology Ano de publicação: 2003 Tipo de documento: Artigo