Primary culture of mammary epithelial cells derived from human breast hypertrophy / 中华整形外科杂志
Chinese Journal of Plastic Surgery
;
(6): 356-359, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-271271
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the primary culture of mammary epithelial cells derived from human breast hypertrophy.</p><p><b>METHODS</b>Mammary epithelial cells of human breast hypertrophy were isolated and cultured by the collagenase digestion method. The morphology observation and identification of the cultured cells were performed by inverted microscope observation, HE staining and cytokeratin immunohistochemical staining.</p><p><b>RESULTS</b>Most of the cultured cells were pebbles-like or polygon under the inverted microscopy. Some had irregular form. They had typical island-like appearance during multiplication with close connection between the cells. The HE staining results showed the cytoplasm was stained pink or lilac, the nucleus was stained bluish violet and was round or oval in shape, with clearly visible chromosomes in dark blue. The cytokeratin immunohistochemical staining demonstrated the tissue-specific expression of cytokeratin 18 in epithelial cells by the cytoplasm stained claybank.</p><p><b>CONCLUSION</b>High purity of primary mammary epithelial cells derived from human breast hypertrophy can be obtained by the collagenase digestion method and conditioned medium.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Mama
/
Células Cultivadas
/
Biologia Celular
/
Células Epiteliais
/
Cultura Primária de Células
/
Hipertrofia
/
Métodos
Limite:
Feminino
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Plastic Surgery
Ano de publicação:
2012
Tipo de documento:
Artigo
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