Analysis of anisodine and its metabolites in rat plasma by liquid chromatography-tandem mass spectrometry / 药学学报
Acta Pharmaceutica Sinica
;
(12): 518-521, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-271415
ABSTRACT
<p><b>AIM</b>To identify anisodine and its metabolites in rat plasma after ingestion of anisodine by combining liquid chromatography and tandem mass spectrometry (LC-MS(n)).</p><p><b>METHODS</b>Plasma samples from rats after a single orally administration of 20 mg anisodine were added with methanol to precipitate protein. Then, it was analyzed by LC-MS(n). Identification and structural elucidation of the metabolites were performed by comparing their changes in molecular masses, retention-times and full scan MS(n) spectra with those of the parent drug and blank plasma.</p><p><b>RESULTS</b>The results revealed that the parent drug and its four metabolites (norscopine, scopine, hydroxyanisodine, N-oxide anisodine) existed in rat plasma.</p><p><b>CONCLUSION</b>This method is sensitive, rapid, simple, and it is suitable for the rapid identification of drug and its metabolits.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plantas Medicinais
/
Derivados da Escopolamina
/
Química
/
Administração Oral
/
Cromatografia Líquida
/
Sensibilidade e Especificidade
/
Ratos Wistar
/
Solanaceae
/
Espectrometria de Massas em Tandem
/
Metabolismo
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2006
Tipo de documento:
Artigo
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