Determination of alkaloids in Radix Aconiti Lateralis Preparata by RP-ion-pair HPLC / 药学学报

ABSTRACT

<p><b>AIM</b>To separate and quantitatively determine six alkaloids aconitine, mesaconitine, hypaconitine, beiwutine, benzoylaconine and benzoylmesaconine in the Chinese traditional medicine Radix Aconiti Lateralis Preparata (Fuzi).</p><p><b>METHODS</b>A RP-ion-pair HPLC method was established. An AichromBond-1 C18 column was used at a column-temperature of 35 degrees C. The mobile phase was CH3CN5 mmol x L(-1) NaH2PO4(5050) containing 7 mmol x L(-1) SDS at a flow-rate of 1.0 mL x min(-1). The detector was set at UV 235 nm.</p><p><b>RESULTS</b>These six alkaloids can be completely separated and determined quantitatively.</p><p><b>CONCLUSION</b>This method is accurate and suitable for the determination of six alkaloids in Fuzi.</p>