Induction of apoptosis of tumor multidrug resistance cell by uvarigrin and its mechanism / 药学学报
Acta Pharmaceutica Sinica
; (12): 252-256, 2006.
Article
em Zh
| WPRIM
| ID: wpr-271465
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To study the effect of uvarigrin on mitochondrial dependent pathway during the apoptosis induced by it in MDR KBv200 cells and their parental sensitive KB cells.</p><p><b>METHODS</b>MTT assay was used to detect the cytotoxic effect of uvarigrin on KBv200 and KB cells. Annexin V FITC staining identified uvarigrin-induced apoptosis in KBv200 and KB cells. These cells underwent incubation with DCFH-DA, or DiOC6, followed by flowcytometry for the measurement of reactive oxygen species (ROS) and mitochondrial membrane potential (deltapsim), respectively. The Western blotting analysis was performed on Caspase-9 activation.</p><p><b>RESULTS</b>Uvarigrin inhibited the growth of KBv200 cells and KB cells in vitro. Most of the uvarigrin-induced cells death was found to be due to apoptosis, as determined by Annexin V FITC staining. During the apoptosis, the level of ROS increased while the level of deltapsim decreased in a time-dependent manner. Uvarigrin triggered Caspase-9 activation.</p><p><b>CONCLUSION</b>Uvarigrin induced apoptosis in KBv200 cells and KB cells probably through a mitochondria-dependent pathway.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Farmacologia
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Fisiologia
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Plantas Medicinais
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Células KB
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Química
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Espécies Reativas de Oxigênio
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Apoptose
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Resistência a Múltiplos Medicamentos
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Resistencia a Medicamentos Antineoplásicos
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Uvaria
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2006
Tipo de documento:
Article