Role of nitric oxide in inhibitory effect of somatic afferent inputs on central pressor response / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 477-508, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-271499
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of nitric oxide in the inhibitory effect of somatic afferent input on the pressor response caused by electrical stimulation of the paraventricular nucleus of the hypothalamus (PVN).</p><p><b>METHODS</b>SD rats anesthetized by urethane were used in the study. Bipolar stainless stimulating electrode was inserted into PVN for electrical stimulation. Multi-barreled micropipettes were used for microinjection of L-NAME or normal saline into the lateral ventricle or amygdala. Deep peroneal nerve (DPN) was stimulated with electrical current pulses of 0.4 mA with duration of 0.5 ms at 4 Hz for 5 min. PVN was stimulated by electrical current pulses of 0.3 mA with duration of 0.5 ms at 80 Hz for 10 sec.</p><p><b>RESULT</b>Electrical stimulation of PVN increased mean arterial pressure. Stimulation of DPN significantly inhibited the pressor response induced by stimulation of PVN (P<0.01), with the inhibitory percentage of 43.27%. Microinjection of L-NAME (0.5 mol/L,10 microl) into the lateral ventricle of brain attenuated the inhibitory effect of DPN. The inhibitory percentage decreased from 47.73% to 12.49% (P<0.05). Microinjection of L-NAME (2 mol/L,100 nl) into amygdala reduced the inhibitory effect of DPN. The inhibitory percentage of stimulating DPN on the pressor response decreased from 50.71% to 25.30% (P<0.05).</p><p><b>CONCLUSION</b>Nitric oxide in the brain and amygdala are involved in the inhibitory effect of somatic afferent input on central pressor response.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Núcleo Hipotalâmico Paraventricular
/
Nervo Fibular
/
Farmacologia
/
Fisiologia
/
Pressorreceptores
/
Pressão Sanguínea
/
Vias Aferentes
/
Ratos Sprague-Dawley
/
Óxido Nítrico Sintase
/
NG-Nitroarginina Metil Éster
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2007
Tipo de documento:
Artigo
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