Mechanism of apoptosis-inducing effects of dopamine on K562 leukemia cells / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 191-195, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-271551
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the mechanism of the apoptosis-inducing effects of dopamine on K562 leukemia cells.</p><p><b>METHODS</b>K562 cells were treated with DP2785, the dopamine receptors were detected with fluorescence spectrophotometer, UV spectrophotometer and fluorescence microscope; the contents of cAMP in K562 cells were measured; and the subtypes of dopamine receptor on K562 cells were analyzed by receptor blocking.</p><p><b>RESULT</b>The existence of dopamine receptors in K562 cells was demonstrated by fluorescence microscopy, UV spectrophotometer and fluorescence spectrophotometer. Dopamine enhanced the contents of cAMP in K562 cells. Dopamine receptors were blocked by both D1 and D2 antagonists.</p><p><b>CONCLUSION</b>D1 and D2 dopamine receptors may be involved in dopamine-induced apoptosis of K562 cells, and dopamine can also increase the contents of cAMP in K562 cells.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Espectrometria de Fluorescência
/
Espectrofotometria Ultravioleta
/
Dopamina
/
Receptores de Dopamina D2
/
Receptores de Dopamina D1
/
Apoptose
/
AMP Cíclico
/
Células K562
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2007
Tipo de documento:
Artigo
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