Analysis on bioactivity of HIV-1 integrase by ELISA method / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 179-184, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-271553
ABSTRACT
<p><b>OBJECTIVE</b>To develop an ELISA-based method for analyzing biologic activities of HIV-1 integrase and for high throughput screening of integrase inhibitors.</p><p><b>METHODS</b>After expression, renaturation and purification of integrase, the bioactivity of integrase and the inhibition of luffin-a were evaluated with an in vitro assay based on biotin-avidin EILSA and chemiluminescent substrates.</p><p><b>RESULT</b>(1) The specific activity of the purified integrase was 54.92 units/mg of protein. (2)IC(50) (concentration causing 50% inhibition of integrase) of luffin-a was (0.63 +/- 0.026) micromol/L.</p><p><b>CONCLUSION</b>The non-radioactive assay can be used for analysis of bioactivities and high throughput screening of inhibitors of HIV-1 integrase.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Especificidade por Substrato
/
Ensaio de Imunoadsorção Enzimática
/
Cinética
/
Catálise
/
Química
/
Integrase de HIV
/
Relação Dose-Resposta a Droga
/
Inibidores Enzimáticos
/
Proteínas Inativadoras de Ribossomos Tipo 1
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2007
Tipo de documento:
Artigo
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