Effects of hydrostatic pressure in physiological range on bladder smooth muscle cells in vitro / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 691-696, 2012.
Article
em Zh
| WPRIM
| ID: wpr-271707
Biblioteca responsável:
WPRO
ABSTRACT
To explore the effects of the physiological range of hydrostatic pressure on human bladder smooth muscle cells (HBSMCs) cultured in vitro, we used a hydrostatic compression device designed in our laboratory into the experiments, which were grouped by varied hydrostatic pressure gradients. Cellular morphology was observed with HE staining; cytoskeleton F-actin, cell cycle, both proliferating cell nuclear antigen (PCNA) and matrix metalloproteinase 7 (MMP-7) were detected respectively with immunofluorescence, flow cytometry and RT-PCR. We found that the proliferation, cytoskeleton and cycle distribution of HBSMCs were not obviously different among the groups of different hydrostatic pressure; however, the mRNA expression of MMP-7 exhibited a trend of first increasing and then declining as the pressure gradually rises. Thus the physiological range of hydrostatic pressure may not have significant influence on proliferation, morphology, skeleton, and cell cycle of HBSMCs, but it may have great effect on the expression of MMP-7.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Fisiologia
/
Bexiga Urinária
/
RNA Mensageiro
/
Células Cultivadas
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Antígeno Nuclear de Célula em Proliferação
/
Metaloproteinase 7 da Matriz
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Biologia Celular
/
Miócitos de Músculo Liso
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Genética
/
Pressão Hidrostática
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2012
Tipo de documento:
Article