Effect of essential oil of Radix Angelicae Dahuricae on beta-endorphin, ACTH, NO and proopiomelanocortin of pain model rats / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of essential oil of Radix Aangelicae Dahuricae (EOAD) on beta-endorphin Adrenocorticotropic hormone(ACTH), Nitric oxide (NO) and Proopiomelanocortin (POMC) of pain model rats induced by formaldehyde.</p><p><b>METHOD</b>beta-endorphin and ACTH were detected with radio-immunity, the amount of masculine cell expression of Proopiomelanocortin mRNA in hypothalamus, and brainstem of pain model was detected with in situ hybridization and NO with chemical method.</p><p><b>RESULT</b>EOAD could increase beta-endorphin, NO and the amount of masculine cell expression of Proopiomelanocortin mRNA significantly in hypothalamus and brainstem of rats.</p><p><b>CONCLUSION</b>EOAD which may be central analgesic, is associated with endogenous opiate-like substance such as POMC and beta-endorphin.</p>