Somatic hybridization between carrot and Bupleurum scorzonerifolium and esteras isoenzyme analysis / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To transfer the effective elements of Bupleurum scorzonerifolium into carrot, and provide theoretical data for the exploitation, improvement and selection of the germplasm of Chinese medicinal plants.</p><p><b>METHOD</b>The protoplasta of Bupleurum scorzonerifolium irradiated by ultraviolet light (UV) at an intensity of 300 microW.(cm2)-1 for 0, 1, 2 min respectively were fused with those of carrot Fisch by PEG method. The regenerated clones, derived form a single fused cell, were examined for their hybrid nature by phenotype and Esterase isoenzyme analysis.</p><p><b>RESULT</b>Nine clones were identified as the somatic hybrids between B. scorzonerifolium and carrot.</p><p><b>CONCLUSION</b>This provides a firm foundation for the further analysis of the main active components saikosaponin of somatic hybrids and the screening out of high-medicine-content hybrid cell lines.</p>