Effects of Different Storage Time and Temperature on Coagulation Function of Fresh Frozen Plasma after Thawing / 中国实验血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the coagulation function changes of fresh frozen plasma storaged at different storage time and temperature after thawing.</p><p><b>METHODS</b>Forty unit of fresh frozen plasma were collected, and thawed at 37 °C for 25 minutes. Each unit was divided into 2 halves one was stored at (4±2)°C for 72 hours and the other one was stored at (25±2) °C for 72 hours. At the time point of 0, 4, 24, 48 and 72 h, thromboela-stogram TEG was measured for all samples. At the the same time, factors V,VII,VIII and IX, APTT and PT were also measured for all the samples. Blood culture for all the samples was used to discover aerobic and anaerobic bacteria.</p><p><b>RESULTS</b>All the samples could form a stable blood clot after thawing for 72 h, and the blood culture results of all samples were negative. Significant changes were observed in ACT and TMA between 0 h and other test time, but there was no difference between 4 °C and 25 °C. The activity of factor V was significantly different between 4 °C and 25 °C after storing for 48 and 72 hours, which was reduced faster at 25 °C.</p><p><b>CONCLUSION</b>Although part of the coagulation factor activity were attenuated after fresh frozen plasma being thawed and stored for 72 hours at different temperature, but all samples can form stable blood clots. Fresh frozen plasma stored for more than 72 hrs after thawing can be used to supplement the coagulation factors to patient.</p>