Apoptosis and its mechanisms of spleen CD4⁺ CD25⁺ regulatory T cells in severe aplastic anemia mouse model / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 931-935, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-272081
ABSTRACT
<p><b>OBJECTIVE</b>To explore the apoptosis and its mechanisms of spleen CD4⁺ CD25⁺ regulatory T (Treg) cells in severe aplastic anemia (SAA) mouse model induced by interferon (IFN-γ) in combination with busulphan (BU).</p><p><b>METHODS</b>The BALB/c female mice SAA model was induced by intraperitoneal injection with IFN-γ and intragastric administration with BU (combined group, n=16), with BU group (n=16), IFN-γ group (n=16) and normal group (n=16) as controls. Spleen Treg cells were purified by using of immunomagnetic beads. Apoptosis was detected by flow cytometry. AKt and TGF-β expression was measured by Western blot.</p><p><b>RESULTS</b>Apoptosis of spleen Treg cells in combined group [(33.21±0.65)%] was significantly higher than that in BU group [(21.58±0.64)%], IFN-γ group [(17.62±1.05)%], and control[(27.38±1.89)%] (P<0.05). A significantly lower expression of AKt and TGF-β protein was also seen in combined group (0.30±0.05 and 0.17±0.05), as compared to the other three groups (P<0.05).</p><p><b>CONCLUSION</b>Excessive apoptosis of Treg cells was found in SAA mouse model, which may be a cause of Treg cells decrease in patients with AA. The down-regulated expression of AKt and TGF-β could play a role in increased apoptosis of Treg cells. Our data may provide a new treatment strategy in AA.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Baço
/
Apoptose
/
Linfócitos T Reguladores
/
Biologia Celular
/
Modelos Animais de Doenças
/
Anemia Aplástica
/
Camundongos Endogâmicos BALB C
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2013
Tipo de documento:
Artigo
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