Amino-terminal pro-brain natriuretic peptide and brain natriuretic peptide measurements under various detection conditions in patients with chronic heart failure / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 695-699, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-272177
ABSTRACT
<p><b>OBJECTIVE</b>To find the potential interference factors for the detection of NT-proBNP and BNP in patients with chronic heart failure.</p><p><b>METHODS</b>EP15-A2 issued by Clinical and Laboratory Standards Institute (CLSI) was employed to compare the precision and accuracy of commercial NT-proBNP and BNP analyzer electrochemiluminescence immunoassay system Cobas E601 and chemiluminescence system ADVIA Centaur. Moreover, NT-proBNP and BNP were detected in different time interval and in different interfered sampling conditions (haematolysis, choloplania, lipemia). NT-proBNP and BNP of 203 patients with heart failure or heart failure complicated with acute cerebral infarction were analyzed to find the deviation caused by patients' endogenous factors.</p><p><b>RESULTS</b>The precision and accuracy were comparable for NT-proBNP and BNP detection using Cobas E601 and ADVIA Centaur (total-CV below 2.9% and 3.5%, the deviation from definite value below 2.38% and 3.91%). The most suitable sample type for NT-proBNP and BNP detection was serum and EDTA-anticoagulant plasma. The detection results of NT-proBNP and BNP were comparable for at least 120 min post sampling and not affected by Hb (2 g/L), DB (428 µmol/L) and chyle (2000 FIU). NT-proBNP was significantly higher in heart failure patients complicated with cerebral infarction (P = 0.003) than in heart failure patients. BNP was significantly higher in heart failure grade III patients complicated with cerebral infarction (P < 0.01).</p><p><b>CONCLUSIONS</b>Cobas E601 and ADVIA Centaur supplied satisfactory detection of NT-proBNP and BNP in patients with chronic heart failure with strong anti-interference capacity. The diagnostic value of NT-proBNP and BNP for chronic heart failure should be analyzed objectively in the presence of complicating diseases.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fragmentos de Peptídeos
/
Padrões de Referência
/
Manejo de Espécimes
/
Sangue
/
Imunoensaio
/
Sensibilidade e Especificidade
/
Peptídeo Natriurético Encefálico
/
Diagnóstico
/
Técnicas Eletroquímicas
/
Insuficiência Cardíaca
Tipo de estudo:
Estudo diagnóstico
/
Guia de Prática Clínica
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Cardiology
Ano de publicação:
2011
Tipo de documento:
Artigo
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