MicroRNA-144 over-expression induced myocytes apoptosis / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 353-357, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-272246
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of microRNA-144 (miR-144) expression on H9C2 (2-1) myocytes.</p><p><b>METHODS</b>MiR-144 was up-regulated in primary cultured H9C2 (2-1) myocytes through transfection. Cells transfected with Lipofectamine(TM) 2000 and its mixture with miRNA synthesized randomly as blank control and negative control respectively. The up-regulation of miR-144 was confirmed by real-time PCR. Cell apoptosis was evaluated by means of CCK-8, Caspase-3 and flow cytometry.</p><p><b>RESULTS</b>Real-time PCR results showed that the miR-144 expression was obviously increased in miR-144 up-regulation group (2178.84 ± 838.52) compared with negative (2.06 ± 0.73) and blank (1.00 ± 0.00) control group (all P < 0.01). The proliferation was lower, the activity of Caspase-3 was elevated and the apoptosis rates were significantly increased in miR-144 up-regulation group compared with negative and blank control group, while no significant difference was found between the latter 2 groups.</p><p><b>CONCLUSION</b>MiR-144 mimics may selectively up-regulate the expression of miR-144 in myocardial cells and consequently promote apoptosis and inhibit proliferation in myocardial cells.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sincalida
/
Transfecção
/
Linhagem Celular
/
Apoptose
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Células Musculares
/
MicroRNAs
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Caspase 3
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Genética
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Cardiology
Ano de publicação:
2011
Tipo de documento:
Artigo
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