Study on the acute toxicity experiment of mice and anti-tumor function in vitro of the qinglongyi / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 887-890, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-272775
ABSTRACT
<p><b>OBJECTIVE</b>To study the anti-tumor effect and acute toxicity in vitro of the separation compositions from qinglongyi.</p><p><b>METHOD</b>The conventional acute toxicity experiments of mice, the MTT [3-(4,5-dimethylthiazol-2 yl)-2,5-diphenylterazolium bromide, MTT] and the SRB (sulforhodamin B) were used to make preliminary selection to qinglongyi and its separation compositions.</p><p><b>RESULT</b>The half-deadly dose (LD50, the half-lethiferous dose) of the chloroform separation composition in qinglongyi was 575.38 mg x kg(-1) (i.g.), and of the acetic ether separation compositions in qinglongyi was 1303.59 mg x kg(-1) (i.g.). From the other parts of separation compositions in qinglongyi, the LD50 were more than 5 g x kg(-1). When acetic ether separation composition of qinglongyi was at 100 microg x mL(-1) the growth inhibitory rate (GIR) was < 50% to the leukaemia cell HL-60 of human. When chloroform separation composition and the acetic ether separation compositions of qinglongyi was at 100 mg x mL(-1), GIR was 52% to the leukaemia cell HL-60, the gastric carcinoma cell BGC-823 and the cervical carcinoma cell Hela of human.</p><p><b>CONCLUSION</b>The separation composition of the chloroform and the acetic ether from qinglongyi have obvious, anti-tumor effect.</p>
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Plantas Medicinais
/
Neoplasias Gástricas
/
Comportamento Animal
/
Medicamentos de Ervas Chinesas
/
Células HeLa
/
Química
/
Células HL-60
/
Concentração Inibidora 50
Limite:
Animais
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2004
Tipo de documento:
Artigo
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