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The effect of sinomenine on cyclooxygenase activity and the expression of COX-1 and COX-2 mRNA in human peripheral monocytes / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 352-355, 2003.
Artigo em Chinês | WPRIM | ID: wpr-272856
ABSTRACT
<p><b>OBJECTIVE</b>To observe in vitro the effect of Sinomenine, a pure alkaloid extracted from the chinese medical plant Sinomenium acutum on the activity of cyclooxygenase (COX-1 and COX-2) and the expression of COX-1 and COX-2 mRNA.</p><p><b>METHOD</b>Mononuclear leukocytes were obtained from healthy adults. Isolated mononuclear leucocytes from human peripheral blood (PBMC) were incubated (1 x 10(6).mL-1) with or without sinomenine (or indomethacin), after incubated for 24 hours at 37 degrees C with 5% CO2; the media were assayed for the PGE2 by radioimmunoassay (RIA). LPS was used to stimulate the monocytes at a concentration of 5 micrograms.mL-1. And by RT-PCR, both COX-1 and COX-2 mRNAs were detected in Mononuclear leukocytes after incubation for different hours with drug (sinomenine or indomethacin) or not.</p><p><b>RESULT</b>LPS (stimulated) induced the production of PGE2 in PBMC increasing with high expression of COX-2 mRNA; sinomenine reduced PGE2 production in LPS stimulated human monocytes more than in non-stimulated human monocytes. In comparative experiments, indomethacin, a non selective COX inhibitor, reduced the production of PGE2 equally in both states. Meanwhile, neither sinomenine(0.1-1 mmol.L-1) nor indomethacin(0.5-10 mumol.L-1) inhibited the expression of both COX-1 and COX-2 mRNAs by RT-PCR with beta-actin as reference.</p><p><b>CONCLUSION</b>In contrast with indomethacin, Sinomenine shows a preferential inhibitory effect on COX-2 over COX-1, These results suggest that Sinomenine is a selective COX-2 inhibitor, which may be directly related to suppressing cyclooxygenase activity.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Plantas Medicinais / Sangue / RNA Mensageiro / Leucócitos Mononucleares / Dinoprostona / Química / Prostaglandina-Endoperóxido Sintases / Sinomenium / Ciclo-Oxigenase 1 Limite: Adulto / Humanos Idioma: Chinês Revista: China Journal of Chinese Materia Medica Ano de publicação: 2003 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Plantas Medicinais / Sangue / RNA Mensageiro / Leucócitos Mononucleares / Dinoprostona / Química / Prostaglandina-Endoperóxido Sintases / Sinomenium / Ciclo-Oxigenase 1 Limite: Adulto / Humanos Idioma: Chinês Revista: China Journal of Chinese Materia Medica Ano de publicação: 2003 Tipo de documento: Artigo