Dexamethasone inhibits osteoblastic differentiation by down-regulation of LIM mineralization protein 1 / 生理学报
Acta Physiologica Sinica
;
(6): 33-37, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-272988
ABSTRACT
To investigate the mechanisms of the inhibition of osteoblastic differentiation by dexamethasone (DEX), the effects of different doses of DEX on the activity of alkaline phosphatase (ALP), the synthesis of osteocalcin (OC) and the expression of collagen type I were observed in the cultured rat osteoblasts. The LIM mineralization protein-1 (LMP-1) mRNA, a positive regulator of osteoblasts, was semi-quantified by RT-PCR. The results showed that a lower dose (10(-9) mol/L) of DEX could enhance the activity of ALP, the synthesis of OC and the expression of collagen type I. However, a higher dose (10(-7) mol/L) of DEX inhibited them and down-regulated the expression of LMP-1 mRNA in osteoblasts. It is suggested that DEX stimulates osteoblast differentiation at lower dose, while at higher dose it inhibits osteoblast differentiation. The inhibitory action of DEX on osteoblast differentiation might be mediated by the down-regulation of LMP-1 mRNA.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoblastos
/
Farmacologia
/
Fatores de Transcrição
/
RNA Mensageiro
/
Dexametasona
/
Regulação para Baixo
/
Diferenciação Celular
/
Células Cultivadas
/
Proteínas de Homeodomínio
/
Biologia Celular
Limite:
Animais
Idioma:
Chinês
Revista:
Acta Physiologica Sinica
Ano de publicação:
2002
Tipo de documento:
Artigo
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