Use of allogenic acellular dermal matrix combined with autologous epidermal cells for the repair of tissue defect / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 412-415, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-273200
ABSTRACT
<p><b>OBJECTIVE</b>To investigate a method for the repair of tissue defect.</p><p><b>METHODS</b>Allogenic acellular dermal matrixes (ADM) were implanted to full-thickness skin defects made on the dorsa of rats. Two weeks later, autologous suspended epidermal cells were transplanted on to the surface of vascularized ADM. Respectively, neoepidermis was macroscopically observed 2, 3, 5 weeks after grafting, and samples were taken to make routine paraffin sections for microscopical examination, and immunohistochemical staining for type IV collagen was also performed.</p><p><b>RESULTS</b>The vascularized ADM could support proliferation and differentiation of epidermal cells, and also could promote the formation of dermal-epidermal junction. Suspended epidermal cells in an artificial culture system in vivo could develop into mature epidermis. The reconstructed skin not only looked like the normal one in appearance in which hair was removed, but also revealed a better function.</p><p><b>CONCLUSIONS</b>Full-thickness skin defect can be repaired by transplanting autologous epidermal cell suspension on to vascularized ADM.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pele
/
Cirurgia Geral
/
Suspensões
/
Transplante Heterólogo
/
Cicatrização
/
Ferimentos e Lesões
/
Transplante de Pele
/
Ratos Wistar
/
Lesões dos Tecidos Moles
/
Transplante de Células
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Stomatology
Ano de publicação:
2005
Tipo de documento:
Artigo
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