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Dynamic observation of lung metastasis model of adenoid cystic carcinoma / 中华口腔医学杂志
Chinese Journal of Stomatology ; (12): 383-385, 2005.
Artigo em Chinês | WPRIM | ID: wpr-273208
ABSTRACT
<p><b>OBJECTIVE</b>To form ACC-M-GFP cells by transfecting pEGFP-1 into ACC-M cells, and to build up lung metastasis model of adenoid cystic carcinoma for dynamic observation in simulated lung environment.</p><p><b>METHODS</b>pEGFP-1 was prepared and then transfected into ACC-M cell lines by using cationic lipid-based gene transfer technique. After successive selection, the ACC-M-GFP cells were collected and inoculated in BALB/C mice. The simulated lung environment was built up, and dynamic observation was performed by laser confocal microscopy within four weeks after transfection.</p><p><b>RESULTS</b>The amount of fluorescent tumor cell colonies and the intensity of fluorescence gradually increased from the second week to the forth week after transfection. In the meantime, only a very small number of tumor cells had the ability to form clones.</p><p><b>CONCLUSIONS</b>We successfully built up a lung metastasis model of adenoid cystic carcinoma for dynamic observation. The model is suitable for capturing and analyzing metastatic tumor cells in early stage.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Neoplasias Bucais / Transfecção / Carcinoma Adenoide Cístico / Linhagem Celular Tumoral / Proteínas de Fluorescência Verde / Genética / Neoplasias Pulmonares / Camundongos Endogâmicos BALB C / Camundongos Nus Limite: Animais / Humanos / Masculino Idioma: Chinês Revista: Chinese Journal of Stomatology Ano de publicação: 2005 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Neoplasias Bucais / Transfecção / Carcinoma Adenoide Cístico / Linhagem Celular Tumoral / Proteínas de Fluorescência Verde / Genética / Neoplasias Pulmonares / Camundongos Endogâmicos BALB C / Camundongos Nus Limite: Animais / Humanos / Masculino Idioma: Chinês Revista: Chinese Journal of Stomatology Ano de publicação: 2005 Tipo de documento: Artigo