In vitro study on cranial neural crest differentiating into ectomesenchymal cell of the first branchial arch by FGF-8 / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 319-322, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-273227
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of FGF-8 on cranial neural crest cell (CNCC) differentiating into ectomesenchymal cell of the first branchial arch, and determine the appropriate dose and stage of CNCC exposure to FGF-8.</p><p><b>METHODS</b>Cranial neural crest explants were cultured in free-serum medium containing modified DMEM/F12 and different doses of FGF-8. The differentiation type of CNCC were determined by in situ hybridization for Hoxa2 and immunocytochemistry for vimentin.</p><p><b>RESULTS</b>Pre-emigrating CNCC demonstrated the negative Hoxa2 stain and positive vimentin stain after treated by 100 ug/FGF-8. Both post-emigrating CNCC group and control group were positive for Hoxa2 and vimentin stain.</p><p><b>CONCLUSIONS</b>On the early stage of CNCC emigration, the first branchial arch phenotype of CNCC could be induced by FGF-8. This experiment could provide in vitro model for study on the mechanism of tooth-jaw regeneration.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Região Branquial
/
Diferenciação Celular
/
Células Cultivadas
/
Nervos Cranianos
/
Biologia Celular
/
Fator 8 de Crescimento de Fibroblasto
/
Mesoderma
/
Camundongos Endogâmicos
/
Crista Neural
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Stomatology
Ano de publicação:
2005
Tipo de documento:
Artigo
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