Lyophilized standards for the calibration of real time PCR assay for hepatitis C virus RNA / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1910-1914, 2006.
Artigo
em Inglês
| WPRIM
| ID: wpr-273388
ABSTRACT
<p><b>BACKGROUND</b>Since October 1997, an international standard for hepatitis C virus (HCV) nucleic acid amplification technology assay, 96/790, has been available. We compared a series of lyophilized standards with known HCV RNA concentrations against the international standard in fluorescence quantitative PCR detection.</p><p><b>METHODS</b>A series of lyophilized sera were calibrated by ROCHE COBAS AMPLICOR HCV Monitor test against the international standard and sent to various manufacturers to analyse the samples using their own kits. Then calibration curves from the series were compared with that obtained from the external standard calibration curve with the manufacture's series.</p><p><b>RESULTS</b>The standard calibration curve with the series of lyophilized serum showed an excellent correlation (R(2) > 0.98), slope and intercept that were similar to those from the manufacture's series. When the standard calibration curve from the series of lyophilized standards were used to define the values of the given sample, lower coefficients of variation between kits from different manufactures were obtained.</p><p><b>CONCLUSION</b>The results showed that the lyophilized standards could be used to setup the standard calibration curve for clinical HCV RNA quantitative PCR detection.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Padrões de Referência
/
Organização Mundial da Saúde
/
Calibragem
/
RNA Viral
/
Reação em Cadeia da Polimerase
/
Hepacivirus
/
Liofilização
/
Genética
Tipo de estudo:
Guia de Prática Clínica
/
Estudo prognóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2006
Tipo de documento:
Artigo
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