Application of BIOMED-2 primers in immunoglobulin gene rearrangement analysis of ocular adnexal lymphoma: a pilot study / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 31-34, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-273427
ABSTRACT
<p><b>OBJECTIVE</b>To assess the practical value of BIOMED-2 primers in the diagnosis of ocular adnexal lymphoma by PCR.</p><p><b>METHODS</b>DNA was extracted from 63 formalin-fixed paraffin-embedded (FFPE) ocular adnexal lymphoma specimens. The DNA quality was evaluated by PCR-based amplification of housekeeping gene beta-actin. IgH(B) and IgK(B) primers of BIOMED-2 standardized clonality analysis system were used to evaluate the immunoglobin gene rearrangements. PCR products were analyzed using capillary electrophoresis and GeneScan software.</p><p><b>RESULTS</b>76.2% (48/63) of FFPE samples produced amplifiable DNA for detection of Ig gene rearrangements.Positive detection rates by BIOMED-2 IgH(B) and IgK(B) primers were 79.2% (38/48) and 68.8% (33/48), respectively, with a combined positive detection rate of 91.7% (44/48).</p><p><b>CONCLUSIONS</b>IgH(B) and IgK(B) primers of BIOMED-2 are suitable for the detection of clonal rearrangements of Ig gene using FFPE specimens of ocular adnexal lymphomas.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Rearranjo Gênico de Cadeia Pesada de Linfócito B
/
Rearranjo Gênico de Cadeia Leve de Linfócito B
/
Projetos Piloto
/
Linfoma de Células B
/
Linfoma Folicular
/
Actinas
/
Inclusão em Parafina
/
Primers do DNA
/
Diagnóstico
Tipo de estudo:
Estudo diagnóstico
Limite:
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Pathology
Ano de publicação:
2010
Tipo de documento:
Artigo
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