RNA interference of HERC4 inhibits proliferation, apoptosis and migration of cervical cancer Hela cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 232-237, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-273782
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of silencing HERC4 on the proliferation, apoptosis, and migration of cervical cancer cell line Hela and the possible molecular mechanisms.</p><p><b>METHODS</b>Three HERC4-specific small interfering RNAs (siRNAs) were transfected into Hela cells, and HERC4 expression in the cells was examined with Western blotting. CCK-8 assay, annexin V-FITC/PI assay, and wound healing assay were used to assess the effect of HERC4 silencing on the proliferation, apoptosis and migration ability of Hela cells. The expression levels of cyclin D1 and Bcl-2 in the cells were detected using Western blotting.</p><p><b>RESULTS</b>Transfection of siRNA-3 resulted in significantly decreased HERC4 protein expression (P<0.01). HERC4 silencing by siRNA-3 markedly suppressed the proliferation and migration of Hela cells, increased the apoptosis rate (P<0.01) and reduced the expression levels of cyclin D1 and Bcl-2 (P<0.01).</p><p><b>CONCLUSION</b>Silencing of HERC4 efficiently inhibits the proliferation, migration, and invasion of Hela cells in vitro, and the underlying mechanisms may involve the down-regulation of cyclin D1 and Bcl-2.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Células HeLa
/
Transfecção
/
Regulação para Baixo
/
Movimento Celular
/
Neoplasias do Colo do Útero
/
Apoptose
/
Proteínas Proto-Oncogênicas c-bcl-2
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Ciclina D1
/
RNA Interferente Pequeno
Limite:
Feminino
/
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2016
Tipo de documento:
Artigo
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