Effects of Different Dose of Cyclophosphamide on Peripheral Blood Cells of Normal ICR Mice / 中国实验血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate effective doses of cyclophosphamide (CTX) for establishment of leukopenia model in ICR mouse.</p><p><b>METHODS</b>Normal ICR mice (n = 96) were divided into CTX(80), CTX(100), CTX(120), CTX(150) and CTX(300) groups, of which mice received CTX intraperitoneally at a dose of 80, 100, 120, or 150 mg/kg once a day for 3 consecutive days, or 300 mg/kg with single injection. The peripheral blood cell counts were detected at various times before and after CTX administration.</p><p><b>RESULTS</b>The peripheral white blood cell nadirs in the mice injected with CTX appeared on day 4 after the first dose of CTX. The peripheral white blood cell nadir in group CTX(100) was 26.7% of the value measured in mice before CTX administrated, and that of group CTX(80) was 35.0%. Higher doses of CTX, however, caused too severity in hematopoietic injury.</p><p><b>CONCLUSION</b>The dose of CTX 100 mg/(kg·d) × 3d is appropriate for leucopenia model of ICR mouse.</p>